HEK|ONE Media System
Improved Transfection and Production
About HEK Cells
The human embryonic kidney cell line HEK293 has the biosynthetic potential for human-like production and is currently used for manufacturing of several therapeutic proteins and viral vectors.
While initially used for adenoviral vector production, HEK293 also became one of the preferred cell lines for transient or stable protein expression. The need for proper protein folding and humanized glycosylation of therapeutic proteins has promoted the production in HEK293 cells.
- Approved by EMA & FDA for human therapeutic production
- Easily grown in serum-free suspension culture
- Capable of producing post-translational modifications
HEK|ONE Media System
Capricorn Scientific has developed high performance media for HEK293 cells in close cooperation with one of our biomanufacturing partners. The media are perfectly suitable for research and further manufacturing.
HEK|ONE media and feed perfectly support cultivation and production processes of stable and transient transfected HEK293 cells in serum-free suspension culture.
- Complete chemically defined, animal component-free, and protein-free system
- Supports growth of high density HEK293 cultures
- Enables sustained, high level expression of recombinant proteins
- Promotes transfection efficiency and production
FEEDING PERFORMANCE
The Viable Cell Density and Viability were tested in a HEK293 feeding regime. The performance of our new HEK|ONE system were compared against two commercially available media.
TRANSFECTION EFFICIENCY BY GFP EXPRESSION
We tested the transfection efficiency of our newly developed HEK|ONE T against two media on the market and a suitable reference in a GFP expression experimental outline using two different cell lines.
HEK|ONE S
HEK|ONE S is a chemically defined, animal component-free, and protein-free medium, supporting cell growth and production of HEK293 cells and other human cell lines. It was developed for high performance cultivation in suspension and expression of recombinant proteins, viruses, and antibodies in stable cell lines or post-transfection.
- Effective medium for stable growth
- Highest viability and production
HEK|ONE S, Chemically-Defined Medium for Stable Expression, without L-Glutamine, with Growth Hormones
HEK|ONE T
HEK|ONE T is a chemically defined, animal component-free, and protein-free medium, designed for efficient transfection and high density suspension culture of HEK293 cells and other human cell lines. It is highly suitable for transient protein expression and viral vector production. HEK|ONE T is compatible with cationic transfection reagents, such as polyethylenimine (PEI). It can be used in research and further
manufacturing.
- Perfect medium for transfection e.g. (PEI)
- Optimized for growth and production processes
HEK|ONE T, Chemically-Defined Medium for Transient Expression, without L-Glutamine, with Growth Hormones
HEK|ONE FEED
HEK|ONE Feed is a chemically defined, animal component-free, and protein-free feeding supplement with highly concentrated nutrients, increasing the productivity of HEK293 cells and other human cell lines. It supports superior production of recombinant proteins and antibodies in suspension culture by maintaining and extending cell growth and production capability. HEK|ONE Feed is compatible with HEK | ONE S as well as HEK|ONE T for stable and transient protein expression.
- Boosts fed-batch processes
- Supports superior yields with human cell cultures
HEK|ONE Feed, Chemically-Defined Feeding Supplement, without L-Glutamine
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