DMEM Advanced High Glucose (4.5 g/l), without L-Glutamine, with Non-Essential Amino Acids, with Sodium Pyruvate
DMEM Advanced (Dulbecco's Modified Eagle Medium) High Glucose is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in DMEM Advanced, with no adaptation, include MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero.
This formulation of DMEM Advanced comes without L-glutamine and is supplemented with non-essential amino acids and sodium pyruvate.