Puromycin Powder is an aminonucleoside antibiotic used for selection of mammalian cell lines expressing the puromycin resistance gene.
Puromycin Powder is active against gram-positive bacteria and various animal and insect cells by inhibiting the peptidyl transfer on prokaryotic and eukaryotic ribosomes. Transfected cells become resistant to puromycin by expression of the pac gene, encoding a puromycin N-acetyl transferase.
Puromycin is used as a selection antibiotic for mammalian cells in the concentration range of 0.5 – 10 µg/ml. For puromycin resistant bacteria that have been transformed with the pac gene, use a concentration of 100 – 125 µg/ml.