RPMI 1640 Advanced, without L-Glutamine, with Non-Essential Amino Acids, with Sodium Pyruvate
RPMI (Roswell Park Memorial Institute) 1640 Advanced is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.
This formulation of Advanced RPMI 1640 comes without L-Glutamine and is supplemented with Non-Essential Amino Acids (NEAA) and with Sodium Pyruvate.
Compared to classic RPMI 1640, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology. Cells successfully cultured in Advanced RPMI 1640, with no adaptation, include Sp2, Vero, Raji, Daudi, and Jurkat.