Easy and reliable

Serum-free media are commonly used in the production of monoclonal antibodies in hybridoma and recombinant myeloma cells. The demands on a medium are very complex, because every cell has its own individual requirements.

Our new media HybridomaPlus and HybridomaPrime have been developed through a complex optimization process. They are suitable for a variety of hybridoma clones.

Protein-free and defined

HybridomaPlus is a completely defined, protein-free medium for the cultivation of various hybridoma cells. The absence of proteins facilitates the antibody purification. HybridomaPlus has been successfully tested in T-flasks, but is also perfectly suitable for use in bioreactors. Full scalability is therefore assured. For applications in bioreactors the addition of Pluronic is recommended.

  • Completely defined
  • Free of animal derived components
  • Protein-free
  • High antibody yield
  • Easy antibody purification

Serum-free and easy

HybridomaPrime is a serum-free medium, optimized to support growth of human, mouse and rat hybridoma as well as myeloma cells. HybridomaPrime contains a defined and complex mixture of amino acids, vitamins and peptides which significantly increases the yield of antibodies. To further increase the growth rate we recommend supplementation with Insulin to improve the specific glucose consumption rate.

  • Serum-free medium with improved properties
  • Minimal adaptation time
  • Suitable for perfusion
  • Free of animal derived components
  • Easy protein A purification

Useful extras

Hybridoma Supplement is a completely defined growth supplement for fusion, selection and cloning of hybridoma cells. Since Hybridoma Supplement must simply be added to the culture medium, there is no need for the more difficult use of feeder cells.

  • Serum-free
  • High cloning efficiency
  • Excellent monoclonal antibody yield
  • Low protein content


Insulin Transferrin Selenium (ITS) is a chemically defined growth supplement for a variety of cell culture applications. These are required for optimum performance of serum-free media. Supplementation of ITS to conventional nutrient media substantially reduces the FBS requirement for routine maintenance of many cell lines such as CHO, Hybridoma, MDCK and Vero.



Test and see for yourself


500 ml Protein-free medium for hybridoma cells, with L-Glutamine
Cat. No.: HY-A; free sample


500 ml Serum-free medium for hybridoma cells, with L-Glutamine
Cat. No.: HP-A; free sample

Hybridoma Supplement

50 ml Serum-free Hybridoma Supplement (10x) Cat. No.: HCS-F,
Special Test Sample Price: 45,00 €/pc. (costs for VAT, freight and dry ice are to be added)

Insulin, Transferrin, Selenium

10 ml ITS (100x), Cat. No.: ITS-H
Special Test Sample Price: 12,50 €/pc. (costs for VAT, freight and dry ice are to be added)