Serum-free media are commonly used in the production of monoclonal antibodies in hybridoma and recombinant myeloma cells. The demands on a medium are very complex, because every cell has its own individual requirements.
HybridomaPlus provides the easiest and safest solution in the production of diverse antibodies with recombinant myeloma lines as well as traditional hybridoma.
Our Hybridoma Supplement replaces the formerly used feeder cells to eliminate disadvantages like overgrowth of newly formed hybridoma, source of contamination, competition for nutrients, and variations in growth factor concentrations.
is a completely defined, protein-free medium for the cultivation of various hybridoma cells. The absence of proteins facilitates the antibody purification. It has been successfully tested in T-flasks and is also perfectly suitable for use in bioreactors. For applications in bioreactors the addition of Pluronic is recommended.
is a completely defined growth supplement for fusion, selection and cloning of hybridoma cells. Since Hybridoma Supplement must simply be added to the culture medium, there is no need for the more difficult use of feeder cells.
substantially reduces the FBS requirement for routine maintenance of many cell lines such as CHO, Hybridoma, MDCK, and Vero.