High titer protein expression in HEK293 cells
Capricorn Scientific has developed high-performance media for HEK293 cells in close cooperation with one of our biomanufacturing partners. The media are perfectly suitable for research and further manufacturing.
Advantages at a glance
The human embryonic kidney cell line HEK293 has biosynthetic potential for human-like production and is nowadays used for manufacturing of several therapeutic proteins and viral vectors. While initially used for adenoviral vector production, HEK293 also became one of the preferred cell lines for transient or stable protein expression. The need for proper protein folding and glycosylation of therapeutic proteins has promoted the production in HEK293.
The product HEK|ONE S is the right cultivation and production medium for stable HEK293 cell lines. It supports stable growth of suspension cells at high viability in seed train culture. HEK|ONE S promotes the production of recombinant proteins and antibodies in suspension culture.
The medium HEK|ONE T was designed for high-density suspension cultures and efficient transfection. Furthermore, it is highly suitable for transient protein expression and viral vector production in HEK293 and other human cell lines. Use the same medium for seeding, transfection, and production.
The HEK|ONE feeding supplement is compatible with HEK|ONE S for recombinant protein production in stable cell lines, as well as with HEK|ONE T for transient gene expression. This highly concentrated nutrient mixture increases the productivity of HEK293 and other human cells. It supports superior production of recombinant proteins and antibodies in suspension culture by maintaining and extending the production capability of HEK293 cells.