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HEK293 Expression

High titer protein expression in HEK293 cells

Capricorn Scientific has developed high-performance media for HEK293 cells in close cooperation with one of our biomanufacturing partners. The media are perfectly suitable for research and further manufacturing. 

 

Advantages at a glance

  • Supports growth of high-density HEK293 cultures
  • Enables sustained, high-level expression of recombinant protein
  • Promotes transfection efficiency and production

 

Download HEK|ONE Brochure

High yield human expression system

The human embryonic kidney cell line HEK293 has biosynthetic potential for human-like production and is nowadays used for manufacturing of several therapeutic proteins and viral vectors. While initially used for adenoviral vector production, HEK293 also became one of the preferred cell lines for transient or stable protein expression. The need for proper protein folding and glycosylation of therapeutic proteins has promoted the production in HEK293.

HEK|ONE S

The product HEK|ONE S is the right cultivation and production medium for stable HEK293 cell lines. It supports stable growth of suspension cells at high viability in seed train culture. HEK|ONE S promotes the production of recombinant proteins and antibodies in suspension culture.

HEK|ONE S, Chemically-Defined Medium for Stable Expression, without L-Glutamine, with Growth Hormones

HEK|ONE S is a serum-free, chemically defined, animal component-free, and protein-free medium for stable Human Embryonic Kidney 293 (HEK293) cell lines.
Cat-No:
HEKS-1000ML
Volume:
1,000 ml
Storage:
+2°C to +8°C
Sterility:
Sterile-filtered
€94.00 View Details

HEK|ONE T

The medium HEK|ONE T was designed for high-density suspension cultures and efficient transfection. Furthermore, it is highly suitable for transient protein expression and viral vector production in HEK293 and other human cell lines. Use the same medium for seeding, transfection, and production.

HEK|ONE T, Chemically-Defined Medium for Transient Expression, without L-Glutamine, with Growth Hormones

HEK|ONE T is a serum-free, chemically-defined, and animal-component-free medium for transient Human Embryonic Kidney 293 (HEK293) cells.
Cat-No:
HEKT-1000ML
Volume:
1,000 ml
Storage:
+2°C to +8°C
Sterility:
Sterile-filtered
€96.90 View Details

HEK|ONE Feed

The HEK|ONE feeding supplement is compatible with HEK|ONE S for recombinant protein production in stable cell lines, as well as with HEK|ONE T for transient gene expression. This highly concentrated nutrient mixture increases the productivity of HEK293 and other human cells. It supports superior production of recombinant proteins and antibodies in suspension culture by maintaining and extending the production capability of HEK293 cells.

HEK|ONE Feed, Chemically-Defined Feeding Supplement, without L-Glutamine

HEK|ONE Feed is a serum-free, chemically-defined feeding supplement for stable and transient HEK293 cells. It can be combined with HEK|ONE S or HEK|ONE T.
Cat-No:
HEKF-1000ML
Volume:
1,000 ml
Storage:
+2°C to +8°C
Sterility:
Sterile-filtered
€149.00 View Details

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