Fed-Batch

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Boost your CHO fed-batch in research and production

 

Our CHO|ONE Media System™ system was designed for small- and large-scale CHO protein expression and features full and easy scalability.

CHO|ONE Media System™

Our CHO|ONE Media System™ is highly suitable for fed-batch cultures. It improves the yield and concentration of recombinant proteins through the optimization of a selection of essential nutrients and growth-promoting factors. CHO|ONE Media System™ was specially designed in close cooperation with our biopharmaceutical cooperation partners using CHO-S, CHO-DG44, and CHO-K1 cell lines. The system keeps the production of stress-related metabolites such as lactate and ammonia at an absolute minimum during the entire procedure of your fed-batch culture.

Advantages at a glance

  • Safe supply with short lead times
  • Animal origin-free, serum-free, protein-free formulations
  • Fast adaptation and scale-up 
  • Individual optimization and customization

CHO|ONE Starter Kit

CHO|ONE Starter Kit contains all media and supplements necessary for the growth of Chinese Hamster Ovary (CHO) cells in batch or fed-batch processes.
Cat-No:
CHO-K1
Storage:
+2°C to +8°C
Sterility:
Sterile-filtered
€182.80 View Details

Each component of the CHO|ONE Media System™ is also available separately:

CHO|ONE A

Our adaptation medium CHO|ONE A allows the cultivation of all recombinant CHO cells in small-scale shaker bottles or spinner flasks. The formulation of this CHO medium simplifies and shortens the time-consuming process of seed and inoculum trains.

CHO|ONE A Adaptation Medium, w/o Insulin, w/o L-Glutamine, with Pluronic™

CHO|ONE A is a protein-free, serum-free, chemically defined medium for the growth of Chinese Hamster Ovary (CHO) cells. It is used for adaptation to CHO|ONE E and seed trains.
Cat-No:
CHOA-500ML
Volume:
500 ml
Storage:
+2°C to +8°C
Sterility:
Sterile-filtered
€48.70 View Details

CHO|ONE E

Our expression medium CHO|ONE E allows the cultivation of recombinant CHO cells for batch and fed-batch cultures in flasks or large-scale bioreactors. This CHO medium is optimized for midsize and large-scale culture in combination with Capricorn Scientifics’ unique supplements CHO|ONE Feed 1 and CHO|ONE Feed 2.

CHO|ONE E Expression Medium, w/o Insulin, w/o L-Glutamine, with Pluronic™

CHO|ONE E is a protein-free, serum-free, chemically defined medium for Chinese Hamster Ovary (CHO) cells. It is used for expression in batch and fed-batch processes.
Cat-No:
CHOE-500ML
Volume:
500 ml
Storage:
+2°C to +8°C
Sterility:
Sterile-filtered
€53.00 View Details

CHO|ONE Feed 1 and Feed 2

The feeding supplements CHO|ONE Feed 1 and CHO|ONE Feed 2 are additives especially matched to the expression medium CHO|ONE E. CHO|ONE Feed 1 contains high concentrations of amino acids, vitamins, salts, trace elements, and a carbon source. CHO|ONE Feed 2 is a balanced formulation of selected amino acids in a concentrated form to perfect the system.

CHO|ONE Feed 1 Supplement for CHO cells, w/o Insulin, w/o L-Glutamine

CHO|ONE Feed 1 is a serum-free feeding supplement, which increases fed-batch productivity with Chinese Hamster Ovary (CHO) cells. It can be combined with CHO|ONE E and CHO|ONE Feed 2.
Cat-No:
CHOF1-100ML
Volume:
100 ml
Storage:
+2°C to +8°C
Sterility:
Sterile-filtered
€24.90 View Details

CHO|ONE Feed 2 Supplement for CHO cells, w/o L-Glutamine

CHO|ONE Feed 2 is a feeding supplement, which increases fed-batch productivity with Chinese Hamster Ovary (CHO) cells. It can be combined with CHO|ONE E and CHO|ONE Feed 1.
Cat-No:
CHOF2-10ML
Volume:
10 ml
Storage:
+2°C to +8°C
Sterility:
Sterile-filtered
€16.30 View Details

Fed-Batch Brochure