CHO Expression: Fed-Batch

Our CHO|ONE Media System™ is highly suitable for fed-batch cultures. It improves the yield and concentration of recombinant proteins through the optimization of a selection of essential nutrients and growth-promoting factors. CHO|ONE Media System™ was specially designed in close cooperation with our biopharmaceutical cooperation partners using CHO-S, CHO-DG44, and CHO-K1 cell lines. The system keeps the production of stress-related metabolites, such as lactate and ammonia, at an absolute minimum during the entire procedure of your fed-batch culture.

Our adaptation medium CHO|ONE A allows the cultivation of all recombinant CHO cells in small-scale shaker bottles or spinner flasks. The formulation of this CHO medium simplifies and shortens the time-consuming process of seed and inoculum trains.

Our expression medium CHO|ONE E allows the cultivation of recombinant CHO cells for batch and fed-batch cultures in flasks or large-scale bioreactors. This CHO medium is optimized for mid-size and large-scale culture in combination with Capricorn Scientific's unique supplements CHO|ONE Feed 1 and CHO|ONE Feed 2.

The feeding supplements CHO|ONE Feed 1 and CHO|ONE Feed 2 are additives specially matched to the expression medium CHO|ONE E. CHO|ONE Feed 1 contains high concentrations of amino acids, vitamins, salts, trace elements, and a carbon source. CHO|ONE Feed 2 is a balanced formulation of selected amino acids in a concentrated form to perfect the system.