CHO|ONE B, Expression Medium with Pluronic™, w/o HT, w/o Insulin, w/o L-Glutamine

Description

CHO|ONE B is a balanced basal medium formulation designed for the cultivation of recombinant CHO cells in fed-batch processes, from spinner flasks to large-scale bioreactors. As the standard representation of the CHO|ONE platform, this chemically defined expression medium provides a well-rounded mixture of nutrients, including balanced levels of amino acids, vitamins, trace metals and glucose. CHO|ONE B delivers robust cell growth and consistent productivity across most CHO clones, including DG44, K1, and S.

As part of the CHO|ONE Media FlexKit, CHO|ONE B enables parallel screening of all CHO|ONE formulations to identify optimal clone performance. Representing the standard CHO|ONE formulation, it supports stable cell proliferation and high productivity without process fine‑tuning. This makes it the ideal starting point for routine fed‑batch production and for clones with average nutrient demands. While it already leads to increased cell growth and protein yield, its full potential is unlocked when combined with CHO|ONE Feeding supplements.

Key Highlights:

• Balanced formulation – Well‑rounded nutrient profile
• Broad applicability – Suitable across most CHO clones
• Robust growth – Consistent performance in batch and fed‑batch systems
• Synergy with CHO|ONE Feeds – Enables straightforward fed‑batch integration