CHO|ONE L, Expression Medium with PluronicTM, w/o HT, w/o Insulin, w/o L-Glutamine

CHO|ONE L is a light nutrient medium with reduced amino acid and glucose levels, universally compatible with all CHO|ONE Feeds and ideal for identifying minimal nutrient requirements or reducing metabolic stress.
Cat-No:
CHOL-500ML
Volume:
500 ml
Storage:
+2°C to +8°C
Sterility:
Sterile-filtered
on request

Description

CHO|ONE L is a light nutrient medium formulation with reduced levels of amino acids and glucose, specifically designed for recombinant CHO cell cultivation in fed-batch systems. This minimal formulation is ideal for identifying a clone’s basal nutrient requirements, reducing metabolic stress, or screening nutrient sensitivities during process development.

As part of the CHO|ONE Media FlexKit, CHO|ONE L allows parallel evaluation of different nutrient densities. The reduced formulation helps pinpoint limiting factors and supports controlled feeding strategies. While CHO|ONE L already achieves sufficient cell growth and protein yield, combining it with CHO|ONE Feeding supplements deploys the full potential of the platform.

Key Highlights:

  • Reduced nutrient levels – Ideal for minimal requirement screening
  • Lowers metabolic stress – Suitable for sensitive clones
  • Enables precise feeding – Helps define tailored supplementation
  • Synergy with CHO|ONE Feeds – Supports precisely controlled, low‑stress feeding strategies for sensitive clones