DMEM High Glucose (4.5 g/l), with L-Glutamine

Dulbecco's Modified Eagle Medium (DMEM) High Glucose is a standard cell culture medium. It supports the growth of numerous cell types. The variety of available formulations suit many applications.
Cat-No:
DMEM-HA
Volume:
500 ml
Storage:
+2°C to +8°C
Sterility:
Sterile-filtered
€6.90

Description

Dulbecco's Modified Eagle Medium (DMEM) is widely used in cell culture as a basal medium. It provides basic nutrients for growth of various mammalian cell lines, especially when supplemented with FBS.

In the past, DMEM was shown to successfully cultivate primary cells, such as fibroblasts, neurons, HUVECs, smooth muscle cells, or even cells lines, such as HEK-293, HeLa, Cos-7, and PC-12.

DMEM comes in various formulations, where the composition of components may differ. The original formulation of DMEM contains 1.0 g/L. This product - DMEM High Glucose - has an increased glucose content of 4.5 g/L and is supplemented with L-glutamine.

Dulbecco's Modified Eagle Medium is based on Eagle's Minimal Essential Medium (EMEM) but has a fourfold higher concentration of amino acids and vitamins.

A very common practice is the supplementation of DMEM with FBS, typically at a concentration of 10%. This is often necessary because the medium does not contain any growth hormones, lipids, or proteins that are essential for cell growth.

DMEM contains a buffer to maintain pH levels and usually comes with phenol red as a pH indicator. Cells cultivated in DMEM, or any other medium containing the bicarbonate buffer system, must be kept in a controlled COenvironment of 5-10%. When phenol red is added, you can monitor pH changes from 6.2 (yellow) to 8.2 (red).

Each high-quality cell culture media by Capricorn Scientific is manufactured according to the originally published formula. We offer modifications essential to the consistent performance and stability of the culture medium. All our classical DMEM variants have been tested to optimally support the growth of your cells.

Components Concentration (mg/L)
› Amino Acids
Glycine 30.00
L-Arginine HCl 84.00
L-Cystine 2 HCl 48.00
L-Glutamine 584.00
L-Histidine HCl H2O 42.00
L-Isoleucine 105.00
L-Leucine 105.00
L-Lysine HCl 146.00
L-Methionine 30.00
L-Phenylalanine 66.00
L-Serine 42.00
L-Threonine 95.00
L-Tryptophan 16.00
L-Tyrosine 2 Na 89.47
L-Valine 94.00
› Vitamins
Choline chloride 4.00
D-Calcium Pantothenate 4.00
Folic Acid 4.00
myo-Inositol 7.20
Nicotinamide 4.00
Pyridoxal HCl 4.00
Riboflavin 0.40
Thiamine HCl 4.00
› Inorganic Salts
CaCl2 2 H2O 265.00
Fe(NO3)3 9 H2O 0.10
KCl 400.00
MgSO4 97.67
NaCl 6400.00
NaHCO3 3700.00
NaH2PO4 109.00
› Other Components
D-Glucose 4500.00
Phenol Red Sodium Salt 15.90