Trypsin-EDTA (0.25%) in HBSS (1x), with Phenol Red
Trypsin-EDTA solutions are used for the detachment of adherent cells from culture surfaces. They are composed of natural porcine pancreas-derived trypsin and EDTA.
The addition of phenol red serves as a pH indicator to adjust to the perfect cell culture conditions.
The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Various formulations should be tested to determine the best product for a specific application.