CHO|ONE FlexKit
Discover the Flexibility Your Clone Needs
The CHO|ONE FlexKits provide a structured, flexible approach to CHO process optimization — from initial medium selection to fine-tuned feeding strategies. The CHO|ONE Media FlexKit enables parallel screening of multiple expression media to identify the optimal nutrient environment for clone-specific growth. Building on these results, the CHO|ONE Feed FlexKit allows systematic evaluation of feed variants to define an optimal fed-batch strategy. Together, they provide an integrated platform that translates screening data into robust and optimized process performance.
CHO|ONE Media FlexKit
The CHO|ONE Media FlexKit provides three optimized formulations — CHO|ONE B, CHO|ONE L and CHO|ONE X — allowing you to screen different nutrient compositions in parallel for your specific production process in batch- and fed-batch systems. A single standard Medium rarely delivers optimal Performance across all applications — Identify the Medium for your specific CHO Cell Line and Process Requirements.
Find the Ideal Nutrient Balance for Your Cells
A light nutrient formulation with reduced amino acid and glucose levels. Ideal for identifying minimal requirements, reducing metabolic stress, or screening nutrient sensitivities.
A basal, well-rounded medium representing the standard CHO|ONE formulation. Provides robust growth and productivity profiles for most CHO cell lines.
A high-nutrient medium with maximized levels of amino acids and glucose. Designed for high-demand cultures, intensified processes, or clones with increased metabolic activity.
Nutrient Level Overview
The CHO|ONE Media FlexKit offers a complete workflow from early screening to fully customized CHO media. Start by testing three nutrient formulations – Basal, Light, and High Nutrient – to evaluate cell growth, productivity, and metabolic performance. Based on your results, our technical experts collaborate with you to tailor the medium to your cell line’s specific needs, including adjustments to glucose, amino acids, vitamins, or trace elements. For enhanced fed-batch processes, the CHO|ONE Feed FlexKit provides complementary feed solutions to systematically optimize nutrient supplementation and support intensified cell culture performance.
The Workflow That Moves You Forward
At Capricorn Scientific, we understand the complexity of media testing for CHO cells in upstream process development. Beyond providing the modular CHOIONE Media and Feed FlexKits, we place strong emphasis on expert consulting throughout the entire process.
Our specialists support you at every stage of media screening and feed strategy development, offering scientific guidance, data-driven recommendations, and optimization expertise. This close collaboration ensures robust decision-making and helps achieve optimal performance, reproducibility, and process reliability tailored to your specific CHO clone.
From Screening to Precision Media — All in One Workflow
CHO|ONE Feed FlexKit
The CHO|ONE Feed FlexKit provides a set of feeding solutions that can be universally combined with all CHO|ONE Media formulations. A unified platform to complete CHO process optimization — from basal media to feeding strategy.
The CHO|ONE Feed FlexKit includes multiple variants of CHO|ONE Feed 1, designed to address different cellular and process requirements and one CHO|ONE Feed 2:
Feed 1A
Basal formulationA robust all-rounder suitable for a wide range of CHO clones and fed-batch processes
Feed 1B
Modified salt compositionOptimized salt concentrations help control osmolality and metabolite accumulation
Feed 1C
Light formulationReduced nutrient load for nutrient-sensitive CHO clones
Feed 2
One standardized versionOffer an additional boost of metabolic support