Aliquoting FBS for Cell Culture

Yes, aliquoting fetal bovine serum (FBS) is strongly recommended. This practice helps preserve the quality of the serum by minimizing freeze-thaw cycles, reducing the risk of contamination, and ensuring consistency in experimental results. It is a crucial step for maintaining the stability and reliability of FBS in cell culture applications.

Additionally, for a more convenient and care-free solution, consider our FBS Minis. These are pre-aliquoted FBS bottles that not only save you time and effort but also come with many other benefits. Enjoy a hassle-free approach to ensure the integrity of your FBS and reproducibility of your experimental results!

Typically, sterile 50-ml conical tubes are used for aliquoting. However, you may use a variety of sterile, sealable reaction tubes suitable for your specific application. These containers should have secure sealing capabilities to prevent contamination and minimize the risk of freezer burn.

Ensure that the vials are labeled with essential information such as date, batch number, and any other relevant details for traceability. Using suitable containers is crucial to maintain the integrity of FBS during storage and handling.

Additionally, consider our FBS Minis as an ideal solution. These pre-aliquoted FBS vials come in orderly 50-ml bottles, providing a convenient and efficient way to handle FBS. With FBS Minis, you eliminate the need for manual aliquoting, ensuring accurate volumes and minimizing the risk of contamination.

When aliquoting, prioritize sterile technique to prevent contamination. Utilize a clean workspace, appropriate personal protective equipment, and work in a controlled environment such as a laminar flow hood. Choose suitable sterile, sealable reaction tubes for aliquoting. Clearly label each aliquot with essential information for traceability and to prevent confusion during experiments.

Additionally, minimize freeze/thaw cycles by aliquoting into smaller volumes, store samples at recommended temperatures, and use separate tools for each aliquoting step to prevent cross-contamination.

Alternatively, you could consider the convenience of pre-aliquoted solutions, such as our FBS Minis, which eliminate manual aliquoting, ensuring accurate volumes and minimizing contamination risks!

The volume of Fetal Bovine Serum (FBS) for each aliquot depends on the final concentration you intend to use in your cell culture medium.

We suggest creating the aliquots in a way that allows you to use each as a single-use aliquot for one bottle of cell culture medium. This practice not only ensures convenient and precise usage but also minimizes the need for multiple freeze/thaw cycles, preserving the quality of the FBS throughout your cell culture experiments.

Many researchers find that aliquoting FBS into volumes of 50 ml is a common and practical approach. Consider your specific experimental needs and the volume required for a single batch of cell culture medium when determining the ideal aliquot size for your FBS.

For added convenience, consider exploring our FBS Minis. These pre-aliquoted FBS vials provide a ready-to-use solution, eliminating the need for manual aliquoting.

It is highly recommended to use a pipette when aliquoting fetal bovine serum (FBS). Using a pipette allows for precise measurement and controlled dispensing of the serum, ensuring accurate volumes for each aliquot.

This precision is crucial for maintaining consistency in cell culture experiments and preventing variations in FBS concentrations. Pouring FBS directly may lead to inaccuracies and uneven distribution, potentially affecting the quality of your samples.

If you are looking to avoid manual aliquoting, consider purchasing our pre-aliquoted FBS Minis. The serum is carefully measured and sealed in sterile conditions, ensuring accuracy and even distribution across each 50-ml bottle.

We recommend thawing fetal bovine serum (FBS) aliquots gradually by transferring them from the freezer to a refrigerator (4 °C). Avoid rapid thawing methods like microwaving or water baths, as these can compromise the quality of the serum.

After thawing, gently mix the FBS by inverting the vial to ensure homogeneity.

Did you know? Using our pre-aliquoted FBS Minis, the bottles can be thawed within 6 hours in the refrigerator, providing a faster turnaround time for your cell culture needs!

When labeling your fetal bovine serum (FBS) aliquots, consider the following key details for traceability and experimental organization:

1. Date of Aliquoting: Clearly mark the date when the FBS aliquots are prepared, to monitor their age and ensure timely usage.
2. Batch Number: Assign a unique batch number to each aliquot for easy tracking and identification.
3. Experiment-Specific Details: Include any experiment-specific information relevant to your workflow, such as project name, researcher name, or other experimental notes.

Ensure that your labeling is clear and accurate (using permanent marker) to prevent any confusion during experiments and to maintain the integrity of your FBS supply.

Want the fail-proof method?
Our FBS Minis offer an additional level of convenience in labeling. They come in practical folding boxes that provide ample space for detailed information.

On the box, you can write important details such as your name, lab group, specific notes, and even keep track of the remaining bottle count!

The box and each bottle inside the box are also labeled with essential information, including product name, lot number, expiry date, and more. This meticulous labeling system on both the box and individual bottles ensures efficient organization and easy retrieval of information when using FBS Minis in your experiments.

Contamination risks during fetal bovine serum (FBS) aliquoting can be mitigated with careful practices.

Firstly, ensure a sterile workspace and use proper personal protective equipment. Work in a laminar flow hood to maintain a controlled environment. Choose sterile containers and equipment, and employ aseptic techniques throughout the aliquoting process. Additionally, minimize the duration of the aliquoting process to reduce exposure.

Want a risk-free option?
Our FBS Minis offer an additional layer of protection against contamination. These pre-aliquoted 50-ml FBS bottles are filled under strict cleanroom conditions in a qualified process. The sterile conditions during manufacturing ensure that the FBS Minis are free from contaminants, providing a convenient and reliable solution for your cell culture needs.

Use our FBS Minis to streamline your workflow and minimize the risk of contamination during aliquoting!

Properly labeling fetal bovine serum (FBS) aliquots is crucial for maintaining organization and traceability. Include key details such as the date of aliquoting, a unique batch number, and experiment-specific information like project name or researcher details. Clear and accurate labeling helps prevent confusion and ensures the integrity of your FBS supply during cell culture experiments.

Consider using our FBS Minis for added convenience in labeling. These pre-aliquoted FBS vials come with a practical folding box that allows you to write essential information like your name, lab group, notes, and keep track of the current bottle count.

Each bottle inside the box is individually labeled with important details such as product name, lot number, and expiry date.

This meticulous labeling system on both the box and individual bottles enhances organization and facilitates easy retrieval of information for your experiments.

Saving time during Fetal Bovine Serum (FBS) aliquoting can be achieved through efficient practices. Firstly, consider using pre-aliquoted solutions like our FBS Minis. These vials are filled under strict cleanroom conditions in a qualified process, eliminating the need for manual aliquoting. With FBS Minis, you save time on preparation and eliminate the risk of contamination, streamlining your cell culture workflow.

Additionally, organize your aliquoting process by setting up a dedicated workspace (laminar flow hood) with all necessary tools and materials readily available and in hand's reach. Properly plan and label aliquots in batches to minimize interruptions and maintain a smooth workflow. By adopting these time-saving strategies, you enhance efficiency and optimize your FBS aliquoting process.