DMEM/F-12, without L-Glutamine, with 15 mM HEPES

DMEM/F-12 is a widely used basal medium for supporting the growth of many different cell types. Due to the composition of DMEM and Ham’s F-12, this formulation offers a rich mixture.
500 ml
+2°C to +8°C


DMEM/F-12 is widely used in cell culture as a basal medium. It provides basic nutrients for growth of various mammalian cell lines, especially when supplemented with FBS.

In the past, DMEM was shown to successfully cultivate primary cells, such as fibroblasts, neurons, HUVECs, smooth muscle cells, or even cells lines, such as HEK-293, HeLa, Cos-7, and PC-12.

DMEM/F-12 is a 1:1 mixture of Dulbecco's Modified Eagle Medium and Ham's F-12 (Ham’s Nutrient Mixture F-12). This formulation of DMEM/F-12 is supplemented with 15 mM HEPES and comes without L-glutamine.

Formulations of DMEM/F-12 with HEPES are usually used for serum-free applications. In these cases, the medium is supplemented with 15 mM HEPES to compensate the missing buffering capactiy.

Dulbecco's Modified Eagle Medium is based on Eagle's Minimal Essential Medium (EMEM) but has a fourfold higher concentration of amino acids and vitamins. Ham's F-12, on the other hand, is based on Ham's F-10 medium.

A very common practice is the supplementation of DMEM with FBS, typically at a concentration of 10%. This is often necessary because the medium does not contain any growth hormones, lipids, or proteins that are essential for cell growth.

DMEM contains a buffer to maintain pH levels and usually comes with phenol red as a pH indicator. Cells cultivated in DMEM, or any other medium containing the bicarbonate buffer system, must be kept in a controlled COenvironment of 5-10%. When phenol red is added, you can monitor pH changes from 6.2 (yellow) to 8.2 (red).

Each high-quality cell culture media by Capricorn Scientific is manufactured according to the originally published formula. We offer modifications essential to the consistent performance and stability of the culture medium. All our classical DMEM variants have been tested to optimally support the growth of your cells.

DMEM/F-12, w/o L-Glutamine, with 15 mM HEPES: Composition

Components Concentration (mg/L)
› Amino Acids
Glycine 18.75
L-Alanine 4.45
L-Arginine HCl 147.50
L-Asparagine H2O 7.50
L-Aspartic Acid 6.65
L-Cysteine HCl H2O 17.56
L-Cystine 2 HCl 31.29
L-Glutamic Acid 7.35
L-Histidine HCl H2O 31.48
L-Isoleucine 54.47
L-Leucine 59.05
L-Lysine HCl 91.25
L-Methionine 17.24
L-Phenylalanine 35.48
L-Proline 17.25
L-Serine 26.25
L-Threonine 53.45
L-Tryptophan 9.02
L-Tyrosine 2 Na 2 H2O 55.79
L-Valine 52.85
› Vitamins
D-Biotin 0.0035
Choline chloride 8.98
D-Calcium Pantothenate 2.24
Folic Acid 2.66
myo-Inositol 12.60
Nicotinamide 2.02
Pyridoxine HCl 0.31
Riboflavin 0.22
Thiamine HCl 2.17
Vitamin B12 0.68
› Inorganic Salts
CaCl2 2 H2O 154.50
CuSO4 5 H2O 0.0013
Fe(NO3)3 9 H2O 0.05
FeSO4 7 H2O 0.417
KCl 311.80
MgCl2 6 H2O 61.20
MgSO4 48.84
NaCl 6996.00
NaHCO3 2438.00
Na2HPO4 71.02
NaH2PO4 54.30
ZnSO4 7 H2O 0.43
› Other Components
D-Glucose 3151.00
HEPES 3574.50
Hypoxanthine 2.1
Linoleic Acid 0.04
Lipoic Acid 0.105
Phenol Red Sodium Salt 8.63
Putrescine 2 HCl 0.081
Sodium Pyruvate 55.00
Thymidine 0.365