DMEM High Glucose (4.5 g/l), without L-Glutamine
DMEM High Glucose without L-Glutamine (Dulbecco's Modified Eagle Medium) is widely used in cell culture as a basal medium. It provides basic nutrients for growth of various mammalian cell lines, especially when supplemented with FBS.
In the past, DMEM was shown to successfully cultivate primary cells, such as fibroblasts, neurons, HUVECs, smooth muscle cells, or even cells lines, such as HEK-293, HeLa, Cos-7, and PC-12.
DMEM comes in various formulations, where the composition of components may differ. The original formulation of DMEM contains 1.0 g/L. This product - DMEM High Glucose - has an increased glucose content of 4.5 g/L and comes without L-glutamine.
Dulbecco's Modified Eagle Medium is based on Eagle's Minimal Essential Medium (EMEM) but has a fourfold higher concentration of amino acids and vitamins.
A very common practice is the supplementation of DMEM with FBS, typically at a concentration of 10%. This is often necessary because the medium does not contain any growth hormones, lipids, or proteins that are essential for cell growth.
DMEM contains a buffer to maintain pH levels and usually comes with phenol red as a pH indicator. Cells cultivated in DMEM, or any other medium containing the bicarbonate buffer system, must be kept in a controlled CO2 environment of 5-10%. When phenol red is added, you can monitor pH changes from 6.2 (yellow) to 8.2 (red).
Each high-quality cell culture media by Capricorn Scientific is manufactured according to the originally published formula. We offer modifications essential to the consistent performance and stability of the culture medium. All our classical DMEM variants that have been tested to optimally support the growth of your cells.
DMEM High Glucose (without L-glutamine): Composition
|› Amino Acids|
|L-Cystine 2 HCl||48.00|
|L-Histidine HCl H2O||42.00|
|L-Tyrosine 2 Na||89.47|
|› Inorganic Salts|
|CaCl2 2 H2O||265.00|
|Fe(NO3)3 9 H2O||0.10|
|› Other Components|
|Phenol Red Sodium Salt||15.90|