RPMI 1640 Medium, with L-Glutamine, with 25 mM HEPES

RPMI 1640 medium is widely suitable for supporting growth of many cell types. Compared to other media, it has a unique formulation comprising glutathione and high concentrations of vitamins.
500 ml
+2°C to +8°C


RPMI 1640 is a growth medium used in different cell culture applications. RPMI stands for Roswell Park Memorial Institute, where it was developed in 1966.

This formulation of RPMI 1640 is supplemented with L-Glutamine and 25 mM HEPES.

RPMI 1640 was developed to support lymphoblastoid cells in suspension cultures. It can also support a wide range of other cell types such as HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. Originally intended to be supplemented with serum, RPMI 1640 has been shown to also support several cell lines in the absence of serum.

Liquid media provide basic nutrients for all cell culture applications. Each of our high-quality cell culture media is manufactured according to the originally published formula or modifications essential to the consistent performance and stability of the culture medium.