Medium 199 with Hanks’ Salts, with L-Glutamine

Medium 199 (M199) was developed for the culture of primary explants. It is suitable for vaccine production, virology and other in vitro cell culture applications.
500 ml
+2°C to +8°C


Medium 199 (M199 medium) with Hanks' Salts was developed in 1950 as a defined nutritional source for cell cultures. It is utilized for cultivation of many cell types as well as vaccine production, virology, and other in vitro cell culture applications.

This formulation of Medium 199 is supplemented with L-glutamine and contains Hanks' salts for use without CO2 (0–2%). When supplemented correctly, Medium 199 has a broad species applicability.

Liquid media provide basic nutrients for all cell culture applications. Each of our high-quality cell culture media is manufactured according to the originally published formula or modifications essential to the consistent performance and stability of the culture medium.